Advancing High-Throughput MS-Based Protein Quantification: A Case Study on Quantifying 10 Major Food Allergens by LC-MS/MS Using a One-Sample Multipoint External Calibration Curve

• DOI: 10.1021/acs.jafc.3c08362
• Publication Date: 2024-03-18T07:24:55Z
• Authors: Shupeng Yang; Haopeng Lin; Peijie Yang; Junhong Meng; Mohamed F. Abdallah; Yingnan Shencheng; Ruohan Li; Jianxun Li; Shuyan Liu; Qianqian Li; Peng Lu; Rong Zhang; Yi Li
• Keywords: Isotope dilution; Sample Preparation; Isotopologue; Sample (material); Robustness

The LC-MS-based method has emerged as the preferred approach for quantifying food allergens. However, preparation of a traditional calibration curve (MSCC) is labor-intensive and error-prone. Here, sensitive robust LC-MS/MS 10 major allergens was developed validated, where one-sample multipoint external (OSCC) employed instead MSCC. By employing multiple isotopologue reaction monitoring (MIRM) technique with only one spiked level in blank, OSCC can be effectively established. Results demonstrate that proposed exhibits excellent performance selectivity, sensitivity, accuracy, precision, comparable to Additionally, this strategy allows isotope sample dilution by less abundant MIRM channel. Moreover, successfully applied investigate contamination commercial products. With its high throughput robustness, MIRM-OSCC-LC-MS/MS methodology many potential applications, especially MS-based protein quantification analysis.