Discovery of New Antimicrobial Metabolites in the Coculture of Medicinal Mushrooms

• DOI: 10.1021/acs.jafc.3c09476
• Publication Date: 2024-03-01T06:49:20Z
• Authors: Linwei Ji; Lingling Tan; Zhaomeng Shang; Wanting Li; Xuhua Mo; Song Yang; Guihong Yu
• Keywords: Medicinal fungi

Bioactivity screening revealed that the antifungal activities of EtOAc extracts from coculture broths Trametes versicolor SY630 with either Vanderbylia robiniophila SY341 or Ganoderma gibbosum SY1001 were significantly improved compared to monocultures. Activity-guided isolation led discovery five aromatic compounds (1–5) broth T. and V. two sphingolipids (6 7) G. SY1001. Tramevandins A–C (1–3) 17-ene-1-deoxyPS (6) are new compounds, while 1-deoxyPS (7) is a natural product. Notably, compound 2 represents novel scaffold, wherein highly modified p-terphenyl bears benzyl substituent. The absolute configurations those elucidated by X-ray diffraction, ECD calculations, analysis physicochemical constants. Compounds 1, 2, 5–7 exhibited different degrees antimicrobial activity, 6 7 against Candida albicans Cryptococcus neoformans comparable fluconazole, nystatin, sphingosine, respectively. Transcriptome analysis, propidium iodide staining, ergosterol quantification, feeding assays showed isolated can extensively downregulate late biosynthetic pathway in C. albicans, representing promising mechanism combat antibiotic-resistant fungi.