Bovine Colostrum miR-30a-5p Targets the NF-κB Signaling Pathway to Alleviate Inflammation in Intestinal Epithelial Cells

• DOI: 10.1021/acs.jafc.3c09856
• Publication Date: 2024-04-12T08:51:57Z
• Authors: Chao Zhang; Jie Zheng; Xueting Han; Jingwen Zhao; Shihui Cheng; Aili Li

Inflammatory bowel disease (IBD) is a common of the digestive system, and an excessive immune response mediated by nuclear factor κ-B (NF-κB) signaling pathway essential etiology. Recent studies have found that bovine milk exosomes can improve intestinal mucosal health delivering microRNA (miRNA), but mechanism action so far unknown. In present study, we analyzed differential expression profiles miRNA in colostrum mature using high-throughput sequencing, based on demonstration inhibit lipopolysaccharide (LPS)-induced epithelial NF-κB inflammatory better than exosomes. The bta-miR-30a-5p, which specifically highly expressed colostrum, was screened, its predicted target gene TRAM to be closely related functional enrichment analysis. Further, used overexpression silencing techniques bta-miR-30a-5p transfection treatment confirmed LPS-induced activation downstream pro-inflammatory expression, while potential gene, TRAM, also suppressed. It hypothesized high targets pathway, may one molecular mechanisms responsible for superior effect resisting attack compared milk.