Screening of IgE-Binding Epitopes of Peach Allergenic Protein Pru p 7 Based on an Immune Microarray Assay

• DOI: 10.1021/acs.jafc.4c00347
• Publication Date: 2024-10-18T13:50:01Z
• Authors: Wenhan Kang; Jiukai Zhang; Ning Yu; Liyang Wei; Ying Chen
• Keywords: Homology

Pru p 7 (also named Peamaclein) is a member of the gibberellin-regulated protein family, which latest foodborne allergenic identified in peach. In this paper, prokaryotic expression and identification were performed, properties, structure, homology analyzed. addition, preliminary screening B-cell linear epitopes was performed by bioinformatics software prediction method, three using slot-blot immune microarray assay combined with an score matrix (P-1, AA16-21, AGYQER; P-2, AA40-46, TYGNKDE; P-3, AA52-59, DLKNSKGN). Moreover, electrostatic potential these analyzed, stability after ultrahigh pressure treatment also verified. Finally, amino acids that play key roles obtained acid mutations. These results may contribute to further understanding prevention peach allergy.