Exopolysaccharides and Surface-Layer Proteins Expressed by Biofilm-State Lactiplantibacillus plantarum Y42 Play Crucial Role in Preventing Intestinal Barrier and Immunity Dysfunction of Balb/C Mice Infected by Listeria monocytogenes ATCC 19115

• DOI: 10.1021/acs.jafc.4c00460
• Publication Date: 2024-04-09T06:06:05Z
• Authors: Jiayi Li; Jiang Yu; Yinglong Song; Sihan Wang; Guangqing Mu; Yanfeng Tuo
• Keywords: Occludin; Lachnospiraceae; Barrier function

Our previous study showed that Lactiplantibacillus plantarum Y42 in the biofilm state can produce more exopolysaccharides and surface-layer proteins a stronger promoting effect on intestinal barrier function than planktonic state. In this study, oral administration of live/pasteurized or L. its metabolites (exopolysaccharides proteins) increased expression Occludin, Claudin-1, ZO-1, MUC2 gut Balb/C mice after exposure to Listeria monocytogenes ATCC 19115 inhibited activation NLRP3 inflammasome pathway, which turn reduced levels inflammatory cytokines IL-1β IL-18 serum mice. Furthermore, abundance beneficial bacteria (e.g., Lachnospiraceae_NK4A136_group Prevotellaceae_UCG-001) while reducing harmful norank_f__Muribaculaceae) mice, line with increase short-chain fatty acids indole derivatives feces Notably, exerted better preventing dysfunction due fact plantarumY42 expressed These results provide data support for use extracted from biofilm-state as functional food ingredients dysfunction.