Establishment of RNAi-Mediated Pest Control Method for Red Imported Fire Ant, Solenopsis invicta

• DOI: 10.1021/acs.jafc.4c00654
• Publication Date: 2024-05-01T20:29:37Z
• Authors: Jin-da Wang; Yao-hui Chen; Ya-xin Zhang; Jin-wen Lin; San-ji Gao; Bao-zhen Tang; You-ming Hou
• Keywords: RNA Silencing

RNAi plays a crucial role in insect gene function research and pest control field. Nonetheless, the variable efficiency of across diverse insects off-target effects also limited its further application. In this study, we cloned six essential housekeeping genes from Solenopsis invicta conducted experiments by orally administering dsRNA. Then, found that mixing with liposomes significantly enhanced targeting for SiV-ATPaseE. Additionally, observed certain lethal effect dsRNA on queens our established system. Furthermore, no strict sequence-related were detected. Finally, large-scale bacteria expressing was successfully confirmed controlling S. invicta. summary, study an system provided template future development nucleic acid drugs based RNAi.