Novel Sesquiterpenoids with Renoprotective Activities from the Fruits of Alpinae oxyphylla as Potent TGF-β1/Smads Phosphorylation Inhibitors

• DOI: 10.1021/acs.jafc.4c01720
• Publication Date: 2024-05-30T14:47:45Z
• Authors: Yue-Tong Zhu; Xiao-Ning Liu; Bo-Tao Lu; Yong-Xian Cheng; Yan-Zhi Wang
• Keywords: R-SMAD

The fruit of Alpinia oxyphylla Miq is an important food spice in southern China and has been used the treatment kidney disorders for centuries. In order to discover natural products with potent renoprotective activities A. provide some references its usage, systematic phytochemical studies were carried out 24 new diverse sesquiterpenoids, including seven guaiane sesquiterpenoids (1–7), 10 eudesmane (9–13, 18, 19, 21–23), six cadinane (31–35 38), eremophilane sesquiterpenoid (40), along known analogues isolated elucidated by analysis spectroscopic data quantum-chemical calculations. Biological evaluation showed that 6 could significantly inhibit expression extracellular matrix components, α-SMA TGF-β1 induced proximal tubular cells (NRK-52e) at low concentrations, 9 also downregulate fibronectin collagen I a concentration-dependent manner, showing their potential renal fibrosis. Further action mechanism study displayed TGF-β1/Smads pathway might be involved antifibrotic effects active 15 43. These suggest may have serve as functional preventing fibrosis-associated diseases.