Potential of Virus-Mediated RNAi of Insect Genes in Plants to Control Aphids

• DOI: 10.1021/acs.jafc.4c09681
• Publication Date: 2025-03-20T18:25:37Z
• Authors: Baozheng Shi; Haifang He; Chenchen Zhao; Caiyan Lei; Jingjing Li; Feng-Ming Yan

Expression of double-stranded RNAs (dsRNAs) in plants is an emerging strategy to efficiently control insects. RNA interference (RNAi)-mediated pest takes advantage that can suppress the expression one or more insect genes encode key proteins. Virus-induced gene silencing (VIGS) a useful tool for plant dsRNAs pests without altering plant's genome. Trehalase (TRE) and chitin synthase (CHS) are very important In this study, we first demonstrated spraying targeting CHS TRE increased mortality rate peach aphid Myzus persicae treated with pathogenic fungus Metarhizium anisopliae. When mpTRE mpCHS were expressed via VIGS, was reduced aphids, their fertility survival rates decreased. These results indicate VIGS-mediated powerful approach effectively aphids had higher when M. anisopliae sprayed.