Flavor Profile of 4-Isothiocyanato-1-butene in Microwave Rapeseed Oil and Its Anti-Inflammatory Properties In Vitro

• DOI: 10.1021/acs.jafc.4c11689
• Publication Date: 2025-04-02T08:44:04Z
• Authors: Xia Xiang; Huihui Liu; Chang Zheng; Nanjie Jiang; Fenghong Huang; Qi Zhou

4-Isothiocyanato-1-butene (4-BITC) is a crucial plant isothiocyanate; however, its flavor profile in microwave rapeseed oil and anti-inflammatory properties have not been elucidated detail. Therefore, this study, the distribution of 4-BITC 45 oils was quantitated using selected ion monitoring, with concentrations ranging from 0.29 to 8.63 mg/kg. The odor activity values ranged 4 123. In lipopolysaccharide (LPS)-induced RAW264.7 cell model, exerted dose-dependent effects, which resulted remarkable differences 20 lipid mediators between LPS groups. Kyoto Encyclopedia Genes Genomes analysis revealed that downregulated proinflammatory oxylipins by modulating CYP, LOX, COX pathways, thereby preventing arachidonic acid metabolism disorders. Molecular docking further confirmed inhibited PI3K/Akt/NF-κB signaling cascade alleviate inflammation.