Comparative Biochemical and Proteomic Analyses of Soybean Seed Cultivars Differing in Protein and Oil Content

• DOI: 10.1021/acs.jafc.5b03196
• Publication Date: 2015-08-03T15:02:25Z
• Authors: Chul Woo Min; Ravi Gupta; So Wun Kim; So Eui Lee; Yong Chul Kim; Dong Won Bae; Won Young Han; Byong Won Lee; Jong Min Ko; Ganesh Kumar Agrawal; Randeep Rakwal; Sun Tae Kim
• Keywords: Proteomics; 2. Zero hunger; 0301 basic medicine; 03 medical and health sciences; Glycine max; Seeds; Plant Oils; Electrophoresis, Polyacrylamide Gel; Mass Spectrometry; Phylogeny; Plant Proteins

This study develops differential protein profiles of soybean (Glycine max) seeds (cv. Saedanbaek and Daewon) varying in protein (47.9 and 39.2%) and oil (16.3 and 19.7%) content using protamine sulfate (PS) precipitation method coupled with a 2D gel electrophoresis (2DGE) approach. Of 71 detected differential spots between Daewon and Saedanbaek, 48 were successfully identified by MALDI-TOF/TOF. Gene ontology analysis revealed that up-regulated proteins in Saedanbaek were largely associated with nutrient reservoir activity (42.6%), which included mainly seed-storage proteins (SSPs; subunits of glycinin and β-conglycinin). Similar results were also obtained in two cultivars of wild soybean (G. soja cv. WS22 and WS15) differing in protein content. Western blots confirmed higher accumulation of SSPs in protein-rich Saedanbaek. Findings presented and discussed in this study highlight a possible involvement of the urea cycle for increased accumulation of SSPs and hence the higher protein content in soybean seeds.