Comparative Study of Nonautolytic Mutant and Wild-Type Strains of Coprinopsis cinerea Supports an Important Role of Glucanases in Fruiting Body Autolysis

• DOI: 10.1021/acs.jafc.5b03962
• Publication Date: 2015-10-09T09:31:54Z
• Authors: Zhonghua Liu; Xin Niu; Jun Wang; Wenming Zhang; Mingmei Yang; Cuicui Liu; Yuanjing Xiong; Yan Zhao; Siyu Pei; Qin Qin; Yu Zhang; Yuan Yu; Sheng Yuan
• Keywords: Autolysis (biology); Pileus; Wild type

Autolysis of Coprinopsis cinerea fruiting bodies affects its commercial value. In this study, a mutant C. that exhibits pileus expansion without autolysis was obtained using ultraviolet mutagenesis. This suggests and involve different enzymes or proteins. Among the detected hydrolytic enzymes, only β-1,3-glucanase activity increased with pilei in wild-type strain, but increase abolished mutant. β-1,3-glucanases plays major role autolysis. Although there are 43 possible β-1,3-glucoside hydrolases genes, 4 known which have products thought to act synergistically degrade β-1,3-glucan backbone cell walls during body autolysis, an unreported gene were upregulated stain suppressed expression these is potentially controlled by single regulatory mechanism.