Water-Soluble Compounds fromLentinula edodesInfluencing the HMG-CoA Reductase Activity and the Expression of Genes Involved in the Cholesterol Metabolism

• DOI: 10.1021/acs.jafc.5b05571
• Publication Date: 2016-02-13T16:50:35Z
• Authors: Alicia Gil-Ramírez; Víctor Caz; Fhernanda R. Smid...; Roberto Martin-He...; Carlota Largo; María Tabernero; Francisco R. Marín; Marcello Iacomini; Guillermo Reglero; Cristina Soler-Rivas
• Keywords: Lentinula; Hydroxymethylglutaryl-CoA reductase

A water extract from Lentinula edodes (LWE) showed HMG-CoA reductase inhibitory activity but contained no statins. NMR indicated the presence of water-soluble α- and β-glucans fucomannogalactans. Fractions containing derivatives these polysaccharides with molecular weight down to approximately 1 kDa still retained their activity. Once digested LWE was applied Caco2 in transport experiments, significant effect noticed on modulation cholesterol-related gene expression. But, when lower compartment monolayer HepG2, some genes were modulated (after 24 h). also administrated normo- hypercholesterolemic mice, lowering serum cholesterol levels observed; reduction triglycerides liver observed. However, supplementation transcriptional profile involved metabolism similarly simvastatin, suggesting that it could hold potential as a hypolipidemic/hypocholesterolemic extract, although further dose-dependent studies should be carried out.