3D Mass Spectrometry Imaging as a Novel Screening Method for Evaluating Biocontrol Agents

• DOI: 10.1021/acs.jafc.5c00349
• Publication Date: 2025-03-31T04:37:46Z
• Authors: Justyna Szulc; Tomasz Grzyb; Beata Gutarowska; Joanna Nizioł; Sumi Krupa; Tomasz Ruman
• Keywords: Mass spectrometry imaging

The aim of this study was to evaluate innovative mass spectrometry imaging (MSI) for determining the metabolic potential selected soil bacteria from genera Bacillus and Priestia in presence phytopathogen Fusarium. This research marks first application direct 3D MSI that visualize interactions between biocontrol agents plant pathogens on agar medium. LARAPPI/CI-3D-MSI (Laser-Assisted Remote Atmospheric Pressure Imaging/Chemical Ionization-3D Mass Spectrometry Imaging) setup provided valuable insights into compounds produced by tested microorganisms, revealing their spatial distributions ability diffuse Subsequently, a Pathway Impact Analysis Metabolites conducted. Ion images based ultrahigh resolution data were obtained, including potentially bioactive compounds. Statistical analysis entire MS set showed metabolites identified licheniformis samples distinctly separated megaterium could be helpful tools screening strains. LARAPPI/CI technique offers significant advantages over existing methods. Further using technology help validate effectiveness various biopreparations contribute enhancing quality biological protection products available market.