Highly Efficient Fructooligosaccharides Production by an Erythritol-Producing Yeast Yarrowia lipolytica Displaying Fructosyltransferase

• DOI: 10.1021/acs.jafc.6b00115
• Publication Date: 2016-04-28T20:23:55Z
• Authors: Lebin Zhang; Jin An; Lijuan Li; Hengwei Wang; Dawen Liu; Ning Li; Hairong Cheng; Zixin Deng
• Keywords: Yarrowia; Aspergillus oryzae; Erythritol

Currently, fructooligosaccharides (FOS) are industrially transformed from sucrose by purified enzymes or fungi cells. However, these methods expensive and time-consuming. An economical approach to producing FOS using erythritol-producing yeast cells was described in this study. Fructosyltransferase Aspergillus oryzae displayed on the cell surface of Yarrowia lipolytica, resulting an engineered strain capable transforming FOS. amount 480 g/L produced within 3 h a solution 800 5 (dry weight, DCW) at pH 6.0 60 °C, with yield 60% total productivity 160 g/(L·h). The pastes erythritol industry can be repeatedly used as whole-cell catalysts least 10 times newly developed approach. This efficient method is attractive for large-scale production sucrose.