Enhancement of Antifungal Activity of Juglone (5-Hydroxy-1,4-naphthoquinone) Using a Poly(d,l-lactic-co-glycolic acid) (PLGA) Nanoparticle System

• DOI: 10.1021/acs.jafc.6b03309
• Publication Date: 2016-09-07T04:11:33Z
• Authors: Tulin Arasoglu; Banu Mansuroglu; Serap Derman; Busra Gumus; Busra Kocyigit; Tayfun Acar; Ismail Kocacaliskan
• Keywords: Juglone; PLGA; Glycolic acid; Zeta potential; Agar dilution

This study aimed to synthesize and characterize juglone-entrapped poly(d,l-lactic-co-glycolic acid) (PLGA) nanoparticles compare the antifungal properties of free juglone with its PLGA nanoparticle formulation for first time. The juglone-loaded prepared using oil-in-water (o/w) single-emulsion solvent evaporation method were characterized by reaction yield (RY), encapsulation efficiency (EE), polydispersity index (PDI), particle size, zeta potential (ZP), FT-IR, in vitro release evaluated their morphological features SEM. had RY, ZP, EE, PDI values 212 nm, 66.91 ± 2.4%, −16.3 0.7 mV, 70.66 3.1%, 0.083 0.024, respectively. In showed a triphasic pattern initial burst followed sustained dormant phase over period, releasing about 72.8% total after 42 days. studies against Aspergillus flavus, Candida albicans, Fusarium spp. agar dilution top methods indicated that juglone-encapsulated was more effective than juglone. method, which applied time on activity, is suitable nanoparticular system based release. Therefore, formulations may be an important tool application many areas beneficial use hydrophobic compounds such as