Development of a qPCR Method for the Identification and Quantification of Two Closely Related Tuna Species, Bigeye Tuna (Thunnus obesus) and Yellowfin Tuna (Thunnus albacares), in Canned Tuna

• DOI: 10.1021/acs.jafc.6b04713
• Publication Date: 2017-01-13T20:25:47Z
• Authors: Daline Bojolly; Périne Doyen; Bruno Le Fur; Urania Christaki; Véronique Verrez-...; Thierry Grard
• Keywords: Yellowfin tuna; Thunnus; Scombridae

Bigeye tuna (Thunnus obesus) and yellowfin albacares) are among the most widely used species for canning purposes. Not only substitution but also mixing of is prohibited by European regulation canned products. However, as juveniles bigeye tunas very difficult to distinguish, unintentional substitutions may occur during process. In this study, two mitochondrial markers from NADH dehydrogenase subunit 2 cytochrome c oxidase II genes were identify tuna, respectively, utilizing TaqMan qPCR methodology. Two different qPCR-based methods developed quantify percentage flesh each can processing. The first one was based on absolute quantification using standard curves realized with these markers; second founded relative universal 12S rRNA gene endogenous gene. On basis our results, we conclude that methodology could be applied authenticate closely related when in a binary mix cans.