Imaging and Detection of Carboxylesterase in Living Cells and Zebrafish Pretreated with Pesticides by a New Near-Infrared Fluorescence Off–On Probe

• DOI: 10.1021/acs.jafc.7b00959
• Publication Date: 2017-05-05T22:06:03Z
• Authors: Dongyu Li; Zhao Li; Weihua Chen; Xingbin Yang
• Keywords: Carboxylesterase; Fluorescence-lifetime imaging microscopy

A new near-infrared fluorescence off-on probe was developed and applied to imaging of carboxylesterase in living HepG-2 cells zebrafish pretreated with pesticides (carbamate, organophosphorus, pyrethroid). The readily prepared by connecting (4-acetoxybenzyl)oxy as a quenching recognizing moiety stable hemicyanine skeleton that can be formed via the decomposition IR-780. response is based on enzyme-catalyzed spontaneous hydrolysis carboxylic ester bond, followed further fragmentation phenylmethyl unit thereby fluorophore release. Compared only existing probe, proposed exhibits superior analytical performance, such emission over 700 nm well high selectivity sensitivity, detection limit 4.5 × 10-3 U/mL. More importantly, cell membrane permeable, its applicability has been successfully demonstrated for monitoring activity pesticides, revealing effectively inhibit carboxylesterase. properties make it great potential use indicating pesticide exposure.