Characterization of Bitter Compounds via Modulation of Proton Secretion in Human Gastric Parietal Cells in Culture

• DOI: 10.1021/acs.jafc.7b01051
• Publication Date: 2017-05-19T22:14:37Z
• Authors: Kathrin I. Liszt; Joachim Hans; Jakob P. Ley; Elke Köck; Veronika Somoza
• Keywords: Parietal cell; Modulation (music)

Humans perceive bitterness via around 25 different bitter receptors. Therefore, the identification of antagonists remains a complex challenge. We previously demonstrated several bitter-tasting compounds such as caffeine to induce acid secretion in stomach and human gastric tumor cell line (HGT-1). Here, results fluorescent-based vitro assay using HGT-1 cells sensory panel testing nine selected potential modulators, with or without theobromine, were compared. Of bitter-modulating tested, eriodictyol, matairesinol, enterolacton, lariciresinol, homoeriodictyol reduced effect on proton by −163 ± 14.0, −152 12.4, −74 16.4, −58 7.2, −44.6 16.5%, respectively, intensity panel. In contrast, naringenin 5,7-dihydroxy-4(4-hydroxyphenyl)chroman-2-one neither caffeine-induced nor showed an perceived Results for theobromine not pronounced those caffeine, but followed similar trend. The demonstrate that is useful tool identify bitter-masking compounds. Nevertheless, necessary quantify potency.