Novel Theaflavin-Type Chlorogenic Acid Derivatives Identified in Black Tea

• DOI: 10.1021/acs.jafc.7b06044
• Publication Date: 2018-03-14T00:04:56Z
• Authors: Shuwei Zhang; Chun Yang; Emmaneul Idehen; Lei Shi; Lishuang Lv; Shengmin Sang
• Keywords: Theaflavin; Chlorogenic Acid; Black tea; Sephadex

Consumption of black tea contributed to many health benefits including the prevention heart disease and certain types cancer. However, chemical composition has not been fully explored. Most studies have examined different interactions between four major catechins, limited investigated interaction catechins other components in tea. In present study, we tested our hypothesis that ortho-dihydroxyl structure chlorogenic acid (CGA) could react with vic-trihydroxy (-)-epigallocatechin 3-gallate (EGCG) (EGC) generate theaflavin-type compounds during fermentation. The reaction CGA EGCG or EGC was catalyzed by horseradish peroxidase (POD) presence H2O2. Two EGCG-CGA EGC-CGA were purified using a Sephadex LH-20 column. Their structures elucidated on basis analysis their MS 1D- 2D-NMR spectroscopic data. Furthermore, existence these two novel characterized LC/MS/MS analysis. We also found had very similar inhibitory effects growth human colon cancer cells theaflavin 3,3'-digallate. These findings shed light bioactive compounds, minor confirmation this type may provide more understanding complexity chemistry.