Laminaria japonica Polysaccharide Inhibits Vascular Calcification via Preventing Osteoblastic Differentiation of Vascular Smooth Muscle Cells

• DOI: 10.1021/acs.jafc.7b06115
• Publication Date: 2018-02-08T08:08:23Z
• Authors: Xue-Ying Li; Qiang-Ming Li; Qing Fang; Xue-Qiang Zha; Li-Hua Pan; Jian-Ping Luo
• Keywords: Male; 0301 basic medicine; Osteoblasts; Plant Extracts; Myocytes, Smooth Muscle; Osteocalcin; Bone Morphogenetic Protein 2; Cell Differentiation; Muscle, Smooth, Vascular; Mice, Inbred C57BL; Mice; 03 medical and health sciences; Polysaccharides; Animals; Humans; Laminaria; Renal Insufficiency, Chronic; Vascular Calcification; Cells, Cultured

This study aimed to investigate the effect and underlying mechanism of a purified Laminaria japonica polysaccharide (LJP61A) on preventing vascular calcification (VC). In adenine-induced chronic renal failure (CRF) mice VC model β-glycerophosphate (β-GP)-induced smooth muscle cells (VSMC) model, LJP61A was found significantly inhibit phenotypes as determined by biochemical analysis von Kossa, alizarin red, immunohistochemical staining. Meanwhile, remarkably up-regulated mRNA levels VSMC related markers down-regulated sodium-dependent phosphate cotransporter Pit-1. addition, could decrease protein core-binding factor-1, osteocalcin, bone morphogenetic 2, receptor activator for nuclear factor-κB ligand, it can increase osteoprotegerin matrix gla protein. These results indicated that ameliorated both in vivo vitro via osteoblastic differentiation VSMC, suggesting might be potential therapeutic agent CRF patients.