Sodium Butyrate Supplementation Alleviates the Adaptive Response to Inflammation and Modulates Fatty Acid Metabolism in Lipopolysaccharide-Stimulated Bovine Hepatocytes

• DOI: 10.1021/acs.jafc.8b01439
• Publication Date: 2018-06-07T09:21:31Z
• Authors: Tianle Xu; Nana Ma; Yan Wang; Xiaoli Shi; Guangjun Chang; Juan J. Loor; Xiangzhen Shen
• Keywords: Sodium butyrate; Fatty Acid Metabolism

This study aimed to evaluate whether sodium butyrate (SB) attenuates the hepatic response LPS-induced inflammation in bovine hepatocytes. Hepatocytes isolated from cows at ∼160 days milk (DIM) were exposed 0.5 mmol/L SB for 18 h as pretreatment. Cells pretreated with used group, and those subjected 4 μg/mL lipopolysaccharide (LPS) challenge 6 (LSB) group. The LPS-challenged hepatocytes showed increases TNF-α IL-6 production culture medium (37 ± 11, P < 0.05); these attenuated by pretreatment LSB group (267 4, 0.05). Compared that LPS-treated cells, phospho-p65 phospho-IκBα protein expression nuclear translocation suppressed when was added. Genes (SREBP1c, SCD1, DGAT1) proteins (SREBP1c SCD1) related fatty acid metabolism upregulated cells compared (P ratios of phospho-AMPKα AMPKα (0.32 0.03 vs 0.70 0.07) phospho-ACCα ACCα decreased (0.81 0.06 2.06 0.16) 0.05) reversed histone H3 deacetylation increased LPS stimulation (0.54 0.02 1.27 0.11, Our results suggest suppresses hepatocyte changes occur during inflammatory response, which is accompanied enhanced synthesis, downregulated oxidation, deacetylation, thus neutralizing negative effects infection.