Cichoric Acid Prevents Free-Fatty-Acid-Induced Lipid Metabolism Disorders via Regulating Bmal1 in HepG2 Cells

• DOI: 10.1021/acs.jafc.8b02147
• Publication Date: 2018-07-23T16:57:50Z
• Authors: Rui Guo; Beita Zhao; Yijie Wang; Dandan Wu; Yutang Wang; Yafan Yu; Yuchen Yan; Wentong Zhang; Zhigang Liu; Xuebo Liu
• Keywords: 0301 basic medicine; Plant Extracts; ARNTL Transcription Factors; Succinates; Hep G2 Cells; Fatty Acids, Nonesterified; Lipid Metabolism; Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; Echinacea; 03 medical and health sciences; Caffeic Acids; Liver; Acetyl Coenzyme A; Non-alcoholic Fatty Liver Disease; Hepatocytes; Humans; PPAR alpha; Signal Transduction

Cichoric acid (CA), a polyphenol component from Echinacea purpurea, exhibits preventive effects on liver lipid-metabolism disorders in obesity. This research aimed to determine the role of circadian rhythm signaling during process CA-attenuated lipid accumulation hepatocytes. In current study, CA treatments improved cell morphology changes and hepatic levels, which were triggered by free fatty acids (2:1, oleate: palmitate) dose-dependent way. Besides, (200 μM) regulated expressions clock genes relatively shallow daily oscillations. Moreover, silencing Bmal1 significantly blocked p-Akt/Akt pathway 80.1% ± 1.5% p-GSK3β/GSK3β 64.7% 2.8% ( p < 0.05). Furthermore, elevated FAS ACC 122.4% 5.6% 114.9% 1.7% protein levels 0.05) 166.5% 18.5% 131.4% 5.5% mRNA Therefore, our results demonstrated that has resistance enhancing Akt/GSK3β pathways modulating downstream related metabolism, indicated might be useful as natural promising nonalcoholic diseases (NAFLD) modulator.