Fish Skin Gelatin Hydrolysate Production by Ginger Powder Induces Glutathione Synthesis To Prevent Hydrogen Peroxide Induced Intestinal Oxidative Stress via the Pept1-p62-Nrf2 Cascade

• DOI: 10.1021/acs.jafc.8b02840
• Publication Date: 2018-10-19T13:13:19Z
• Authors: Liufeng Zheng; Hongkui Wei; Huichao Yu; Qian Xing; Yi Zou; Yuanfei Zhou; Jian Peng
• Keywords: Fish Proteins; 0301 basic medicine; Cell Survival; NF-E2-Related Factor 2; Glutamate-Cysteine Ligase; RNA-Binding Proteins; Cichlids; Hydrogen Peroxide; Glutathione; Peptide Transporter 1; Intestines; Oxidative Stress; 03 medical and health sciences; Zingiber officinale; Biocatalysis; Animals; Gelatin; Humans; Intestinal Mucosa; Peptides; Peptide Hydrolases; Plant Proteins

Gelatin hydrolysate was reported to contain large amounts of biologically active peptides with excellent antioxidant properties. However, its inducement response within cells and the underlying molecular mechanism are far from clear. Here, gelatin Nile tilapia skin hydrolyzed by ginger protease produce hydrolysate, three fish fractions (FSGHFs) were obtained ultrafiltration. Fractionation increased free radical scavenging capacity FSGHFs, particularly FSGHF3, which showed lowest weight (below 1000 Da). Furthermore, FSGHF3 treatment prior H