Effects of Flavored Nonalcoholic Beverages on Transcriptional Activities of Nuclear and Steroid Hormone Receptors: Proof of Concept for Novel Reporter Cell Line PAZ-PPARg

• DOI: 10.1021/acs.jafc.8b05158
• Publication Date: 2018-11-05T15:36:05Z
• Authors: Peter Illés; Aneta Grycová; Kristýna Krasulová; Zdeněk Dvořák
• Keywords: Transcriptional Activation; 0301 basic medicine; Receptors, Thyroid Hormone; Prostaglandin D2; Cell Line; 3. Good health; Beverages; Flavoring Agents; PPAR gamma; 03 medical and health sciences; Receptors, Glucocorticoid; Receptors, Androgen; Humans; Receptors, Calcitriol

We developed and characterized a novel human luciferase reporter cell line for the assessment of peroxisome proliferator-activated receptor γ (PPARγ) transcriptional activity, PAZ-PPARg. The activity induced by PPARγ endogenous agonist 15d-PGJ2 prostaglandin PGD2 reached mean values (87.9 ± 14.0)-fold (89.6 19.7)-fold after 24 h exposure to 40 μM 70 PGD2, respectively. A concentration-dependent inhibition 15d-PGJ2- PGD2-induced was observed application T0070907, selective antagonist PPARγ, which confirms specificity response both agonists. PAZ-PPARg line, along with lines activities thyroid (TR), vitamin D3 (VDR), androgen (AR), glucocorticoid (GR), were used screening 27 commonly marketed flavored nonalcoholic beverages their possible disrupting effects. Our findings indicate that some examined have potential modulate VDR, AR.