Ursolic Acid Isolated from the Leaves of Loquat (Eriobotrya japonica) Inhibited Osteoclast Differentiation through Targeting Exportin 5

• DOI: 10.1021/acs.jafc.8b06954
• Publication Date: 2019-03-04T09:50:50Z
• Authors: Hui Tan; Chong Zhao; Qinchang Zhu; Yoshinori Katakura; Hiroyuki Tanaka; Koichiro Ohnuki; Kuniyoshi Shimizu
• Keywords: Eriobotrya; Ursolic acid

One of the conventional strategies for treating osteoporosis is to eliminate multinucleated osteoclasts that are responsible bone resorption. Our previous study revealed ursolic acid, isolated from leaves loquat used as tasty tea in Japan, suppressed osteoclastogenesis. We confirmed acid exhibited osteoclast differentiation inhibitory activity with an 50% concentration (IC50) value 5.4 ± 0.96 μM. To disclose its mechanism action, this first uses polymer-coated magnetic nanobeads identify potential target proteins. As a result, we identified nuclear exporter protein named exportin 5 (XPO5). Further studies demonstrated knockdown XPO5 significantly blocks ( P < 0.01). Expression profiling mature microRNAs cells downregulation by small interfering RNA or could downregulate expression microRNA let-7g-5p during Collectively, our findings suggest inhibits through targeting XPO5, which provides further evidence healthy function tea. This also new insights into role and mediated treatment resorption diseases.