CsFEX, a Fluoride Export Protein Gene from Camellia sinensis, Alleviates Fluoride Toxicity in Transgenic Escherichia coli and Arabidopsis thaliana

• DOI: 10.1021/acs.jafc.9b00509
• Publication Date: 2019-05-06T18:19:06Z
• Authors: Jiaojiao Zhu; Anqi Xing; Zichen Wu; Jing Tao; Yuanchun Ma; Bo Wen; Xujun Zhu; Wanping Fang; Yuhua Wang
• Keywords: 0301 basic medicine; Fluorides; 03 medical and health sciences; Gene Expression Regulation, Plant; Arabidopsis; Escherichia coli; Biological Transport; Plants, Genetically Modified; Camellia sinensis; Plant Proteins; 3. Good health

A fluoride export gene ( CsFEX) was newly found and isolated from Camellia sinensis, its functions in detoxifying F were investigated transgenic Escherichia coli Arabidopsis thaliana. CsFEX contains two crcB domains, which is the typical structure plants. The expression of C. sinensis tissue-specific related to maturity leaves, significantly induced by treatments different tissues particularly leaves. Additionally, growth E. coli, A. thaliana can all be inhibited treatment. However, CsFEX-overexpression increased with lower content under treatment compared control. Similarly, germination enhanced wild type. relieves toxicity alleviating accumulation.