In Vitro and in Vivo Studies on the Angiotensin-Converting Enzyme Inhibitory Activity Peptides Isolated from Broccoli Protein Hydrolysate

• DOI: 10.1021/acs.jafc.9b01137
• Publication Date: 2019-06-03T19:02:31Z
• Authors: Yali Dang; Tingyi Zhou; Li Hao; Jinxuan Cao; Yangying Sun; Daodong Pan
• Keywords: IC50; Digestion; Pepsin

In the present study we purified and identified peptides from broccoli protein hydrolysates evaluated their angiotensin-converting enzyme (ACE) inhibitory activity in vitro hypotensive effect vivo. Three ACE were isolated as IPPAYTK, LVLPGELAK, TFQGPPHGIQVER, IC50 values 23.5, 184.0, 3.4 μM, respectively. We then investigated of gastrointestinal digestion on activity. detected almost two times peptide LVLPGELAK following simulated than prior to digestion. LVLPGE LAK, novel exhibiting high activity, discovered possessed 13.5 48.0 The was assessed after oral administration spontaneous hypertensive rats (SHRs). found that LAK demonstrated a significant Protein may thus constitute potential antihypertensive source.