Characterization and Inception of a Triterpenoid Astrakurkurol, as a Cytotoxic Molecule on Human Hepatocellular Carcinoma Cells, Hep3B

• DOI: 10.1021/acs.jafc.9b01203
• Publication Date: 2019-06-20T10:17:42Z
• Authors: Sudeshna Nandi; Swarnendu Chandra; Rimpa Sikder; Saurav Bhattacharya; Manisha Ahir; Debanjana Biswal; Arghya Adhikary; Nikhil Ranjan Pra...; Tapan Kumar Lai; Michael G. B. Drew; Krishnendu Acharya
• Keywords: Viability assay

Mushrooms are customary influential sources of pharmaceutically active metabolites. Usually lanostane-type triterpenoids from mushrooms had prospective for cancer disease treatments. Recently, a triterpenoid, astrakurkurol obtained the fresh basidiocarps edible mushroom Astraeus hygrometricus, drew attention as new cytotoxic therapeutic. The structural stability this triterpenoid been established with amalgamation density functional theory (DFT) calculations and study single-crystal X-ray diffraction. To successfully manifest potent therapeutics, wide apprehension on molecular cellular mechanisms underlying their action is prerequisite. On account, our was directed to scrutinize influence human hepatocellular cell model Hep3B. Encapsulating all experimental facts revealed that significantly decreased viability in concentration-dependent manner. This effect unveiled be apoptosis, documented by DNA fragmentation, chromatin condensation, nuclear shrinkage, membrane blebing, imbalance cycle distribution. Astrakurkurol persuaded expression death receptor associated proteins (Fas), which triggered caspase-8 activation following tBid cleavage. Moreover, mediated ROS generation, mitochondrial dysfunction activated apoptotic events. cytotoxicity based caspase-8-mediated intrinsic pathway inhibition at Akt NF-κB pathway. also inhibited migration Hep3B cells, indicating its antimigratory potential. These findings led us introduce feasible natural source safer drug against carcinoma.