Comparative Lipid Profile Analysis of Four Fish Species by Ultraperformance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry

• DOI: 10.1021/acs.jafc.9b03303
• Publication Date: 2019-07-22T19:40:08Z
• Authors: Xuesong Wang; Hongwei Zhang; Yu Song; Peixu Cong; Zhaojie Li; Jie Xu; Changhu Xue
• Keywords: Phosphatidylethanolamine

A high-throughput lipid analysis method was established to comprehensively investigate the profiles of three marine (Scomberomorus niphonius, Scophthalmus maximus, and Oncorhynchus keta) one freshwater (Ctenopharyngodon idellus) fish species using ultraperformance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. Over 700 molecular from 12 major subclasses were identified. Glycerolipids (73.7–85.6%) phospholipids (PLs, 13.7–25.6%) dominant components in total lipids. Polyunsaturated fatty acid PLs, such as phosphatidylethanolamine (PE, 16:0–22:6), PE (18:1–22:6), phosphatidylcholine (16:0–22:6), PLs. The composition (mainly C22:5) significantly different that C. idellus C20:4 C20:5). unique long-chain base sphingolipids found d19:3). These bioactive lipids proposed be potential biomarkers for distinguishing evaluating nutritional values.