MiR-16a Regulates Milk Fat Metabolism by Targeting Large Tumor Suppressor Kinase 1 (LATS1) in Bovine Mammary Epithelial Cells

• DOI: 10.1021/acs.jafc.9b04883
• Publication Date: 2019-09-23T04:09:15Z
• Authors: Zhi Chen; Shuangfeng Chu; Xiaolong Wang; Yujia Sun; Tianle Xu; Yongjiang Mao; Juan J. Loor; Zhangping Yang
• Keywords: Bovine milk; Mammary tumor

Milk contains a number of beneficial fatty acids including short and medium chain unsaturated conjugated nonconjugated acids. In this study, microRNA sequencing mammary tissue collected in early-, peak-, mid-, late-lactation periods was performed to determine the miRNA expression profiles. miR-16a one differentially expressed selected for in-depth functional studies pertaining acid metabolism. The mimic impaired fat metabolism [triacylglycerol (TAG) cholesterol] while knock-down promoted vitro bovine epithelial cells (BMECs). addition, work with BMECs also revealed that had negative effect on cellular concentration cis 9-C18:1, total C18:1, C20:1, C22:1 long-chain polyunsaturated Therefore, these data suggesting extend discovery key role mediating adipocyte differentiation. Through combination bioinformatics analysis, target gene 3' UTR luciferase reporter assays, western blotting, we identified large tumor suppressor kinase 1 (LATS1) as miR-16a. Transfection siRNA-LATS1 into led increases TAG, cholesterol, concentrations, positive LATS1 cell summary, suggest regulates biological processes associated intracellular synthesis through LATS1. These provide theoretical experimental framework further clarifying regulation lipid dairy cows.