Strategies for UHPLC-MS/MS-Based Analysis of Different Classes of Acyl Chain Oxidation Products Resulting from Thermo-Oxidation of Sitostanyl Oleate

• DOI: 10.1021/acs.jafc.9b05197
• Publication Date: 2019-10-02T19:24:25Z
• Authors: Birgit Scholz; Veronika Stiegler; Wolfgang Eisenreich; Karl-Heinz Engel
• Keywords: Hot Temperature; Molecular Structure; Tandem Mass Spectrometry; 04 agricultural and veterinary sciences; 0405 other agricultural sciences; Oxidation-Reduction; Chromatography, High Pressure Liquid; Oleic Acid

Phytostanyl fatty acid esters, which are being added to a variety of foods owing their cholesterol-lowering properties, susceptible thermally induced oxidation reactions. Using sitostanyl oleate as an example, we, in this study, aimed at developing strategies for the detection and quantitation acyl chain products (ACOPs). The elaborated ultra high performance liquid chromatography-mass spectrometry MS/MS-based approach involves (i) derivatizations hydroxy oxo groups, (ii) combined use atmospheric pressure chemical ionization electrospray techniques, (iii) establishment selective multireaction monitoring transitions. These allow identification broad spectrum nonpolar short-chain well polar short- long-chain hydroxy, epoxy, ACOPs. Semiquantitative assessments showed that 9,10-epoxystearate was predominating ACOP resulting from thermo-oxidation 180 °C 30 min. analytical paves way narrow existing knowledge gap on fate phytostanyl esters upon heat treatments.