Imaging Switchable Protein Interactions with an Active Porous Polymer Support

• DOI: 10.1021/acs.jpcb.0c01807
• Publication Date: 2020-05-22T10:41:43Z
• Authors: Chayan Dutta; Logan D. C. Bishop; Jorge Zepeda O; Sudeshna Chatterjee; Charlotte Flatebo; Christy F. Landes
• Keywords: Protein Adsorption; Fluorescence Correlation Spectroscopy

Mechanistic details about how local physicochemistry of porous interfaces drives protein transport mechanisms are necessary to optimize biomaterial applications. Cross-linked hydrogels made stimuli-responsive polymers have potential for active capture and release through tunable steric chemical transformations. Simultaneous monitoring dynamic changes in both interfacial polymer structure is an experimental challenge. We use single-particle tracking (SPT) fluorescence correlation spectroscopy Super-resolution Optical Fluctuation Imaging (fcsSOFI) relate the switchable size a pH-responsive hydrogel properties model protein, lysozyme. SPT analysis reveals reversible switching dynamics at response pH changes. fcsSOFI allows us heterogeneity pores distribution confined diffusion adsorption/desorption. find that physicochemical dictates confinement desorption dynamics, particularly conditions which swollen.