Continuous Uptake or Saturation—Investigation of Concentration and Surface-Packing-Specific Hemin Interaction with Lipid Membranes

• DOI: 10.1021/acs.jpcb.8b03327
• Publication Date: 2018-07-09T05:06:56Z
• Authors: R. P. Giri; M. K. Mukhopadhyay; U. K. Basak; A. Chakrabarti; M. K. Sanyal; B. Runge; B. M. Murphy
• Keywords: Hemin; Saturation (graph theory)

Drug design and targeted delivery in cells serve as a flourishing area not only for scientific inquiry, owing to numerous clinical applications, but also understanding cell interaction with exogenous materials. The membrane localization of heme its analog hemin, one the most biologically relevant planar organic molecule, is very important understand molecular mechanism intercalation adsorption this cytotoxic molecule after dissociation from proteins such hemoglobin. Herein, we investigate differential behavior hemin on soft surfaces phospholipids by synchrotron-based X-ray scattering techniques, Langmuir monolayer measurements, dynamics simulation. A continuous uptake subphase into and/or surface have been witnessed strong packing-specific manner. Competitive interactions between hemin-membrane hemin–hemin are proposed be responsible critical concentration. Up limit, possible beyond that dominates, effectively reducing membrane. This structural model hemin-uptake process can generalized transport across membranes development new drugs.