Omics and Transgenic Analyses Reveal that Salvianolic Acid B Exhibits its Anti-Inflammatory Effects through Inhibiting the Mincle-Syk-Related Pathway in Macrophages

• DOI: 10.1021/acs.jproteome.1c00325
• Publication Date: 2021-06-03T12:04:16Z
• Authors: Jia Li; Ya-Hui Chen; Lan-Zhu Li; Feizuo Wang; Wei Song; Raphael N. Alolga; Wei Zhou; Heming Yu; Feng-Qing Huang; Xiaojian Yin
• Keywords: IκBα; Salvia miltiorrhiza

Salvianolic acid B (Sal B), the main water-soluble compound in Salvia miltiorrhiza, is known to exhibit anti-inflammatory activity, however, underlying mechanism(s) not completely uncovered. In this study, Sal inhibited lipopolysaccharide (LPS)-induced M1 activation and promoted transformation of macrophages from M1- M2-type polarization. The altered lipid profiles LPS-induced RAW 264.7 were partly restored by treatment. At proteomic level, a total 5612 proteins identified 432 significantly changed under LPS differential classified into four clusters according their expression level blank, LPS, groups. Cluster IV including Kif14, Mincle, Sec62 recovered almost normal levels Use knockdown Mincle or picetannol (inhibitor Syk) led significant reductions gene expressions IL-1β, iNOS, IL-12 release NO. converse was, observed for overexpressed Mincle. addition, LPS- trehalose-6,6-dibehenate-induced phosphorylation Syk PKCδ was decreased These results suggest that inhibition inflammation might be through Mincle-Syk-PKCδ signaling pathway.