Proteomic Analysis of Whole Blood Using Volumetric Absorptive Microsampling for Precision Medicine Biomarker Studies

• DOI: 10.1021/acs.jproteome.1c00971
• Publication Date: 2022-02-15T12:17:16Z
• Authors: Mark P. Molloy; Cameron Hill; Matthew B. O’Rourke; Jason Chandra; Pascal Steffen; Matthew J. McKay; Dana Pascovici; Ben R. Herbert
• Keywords: Proteome; Phlebotomy; Biomarker Discovery; Biorepository

Microsampling of patient blood promises several benefits over conventional phlebotomy practices to facilitate precision medicine studies. These include at-home collection, supporting telehealth monitoring, minimal postcollection processing, and compatibility with nonrefrigerated transport storage. However, for proteomic biomarker studies, mass spectrometry whole has generally been avoided in favor using plasma or serum obtained from venepuncture. We evaluated the use a volumetric absorptive microsampling (VAMS) device as sample preparation matrix enable LC-MS analyses dried blood. demonstrated detection robust quantitation up 1600 proteins single-shot shotgun-LC-MS analysis blood, greatly enhancing proteome depth compared undepleted plasma. Some not previously reported were detected this approach. Various washing reagents used demonstrate that can be preferentially removed VAMS devices prior downstream analyses. provide demonstration archival frozen cell pellets housed under long-term storage (exceeding 5 years) are compatible potential biobank repositories. demonstrations important steps establishing viable workflows underpin large-scale Data available via ProteomeXchange identifier PXD028605.