White-Nose Syndrome Disrupts the Splenic Lipidome of Little Brown Bats (Myotis lucifugus) at Early Disease Stages

• DOI: 10.1021/acs.jproteome.2c00600
• Publication Date: 2022-12-08T15:08:30Z
• Authors: Evan L. Pannkuk; Nicole A. S.-Y. D...; Shivani Bansal; Sunil Bansal; Yvonne A. Dzal; Quinn E. Fletcher; Kaleigh J. O. Nor...; Albert J. Fornace; Craig K. R. Willis
• Keywords: Myotis lucifugus; Lipidome; Eptesicus fuscus

White-nose syndrome (WNS)-positive little brown bats (Myotis lucifugus) may exhibit immune responses including increased cytokine and pro-inflammatory mediator gene levels. Bioactive lipid mediators (oxylipins) formed by enzymatic oxidation of polyunsaturated fatty acids can contribute to these responses, but have not been investigated in WNS pathophysiology. Nonenzymatic conversion also occur due reactive oxygen species, however, enantiomeric isomers will lack the same signaling properties. In this study, we performed a series targeted lipidomic approaches on laboratory Pseudogymnoascus destructans-inoculated assess changes their splenic lipidome, formation at early stages WNS. Hepatic lipids previously identified were resolved higher structural detail. We compared WNS-susceptible M. lucifugus WNS-resistant big bat (Eptesicus fuscus). Altered levels only observed lucifugus. Differences free included both omega-3 omega-6 compounds. Increased an monohydroxy DHA mixture found, suggesting nonenzymatic formation. Changes hepatic confined constituents. Together, results suggest that oxidative stress, inflammatory response, is occurring precedes fat depletion. These data submitted metabolomics workbench assigned study number ST002304.