Oral Squamous Cell Carcinoma Lipid Evaluation in Gingiva Tissue Stored in TRIzol via Shotgun Lipidomics and MALDI Mass Spectrometry Imaging

• DOI: 10.1021/acs.jproteome.3c00216
• Publication Date: 2023-10-13T13:42:40Z
• Authors: Ricardo Alves Ber...; Charles Ivo de Ol...; Jussara Valente R...; Nádia do Lago Costa; Virgílio Moreira ...; Carlos Arterio Sorgi; Christian Janfelt; Boniek Gontijo Vaz; Andréa Rodrigues ...
• Keywords: Mass spectrometry imaging; Biomarker Discovery; Trizol; Cancer Biomarkers

Oral squamous cell carcinoma (OSCC) is the prevalent type of oral cavity cancer, requiring precise, accurate, and affordable diagnosis to identify disease in early stages, Comprehending differences lipid profiles between healthy cancerous tissues encompasses great relevance identifying biomarker candidates enhancing odds successful cancer treatment. Therefore, present study evaluates analytical performance simultaneous mRNA extraction gingiva tissue from patients diagnosed with OSCC preserved TRIzol reagent. The data was analyzed by partial least-squares discriminant analysis (PLS-DA) confirmed via matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). solution linear a range 330 2000 ng mL–1, r2 > 0.99, intra interday precision accuracy <15%, absolute recovery values ranging 90 110%. most important lipids for tumor classification were evaluated MALDI-MSI, revealing that responsible distinguishing group are more contrast group. results exhibit possibilities do transcriptomic lipidomic analyses same sample point out related presence OSCC.