Targeted Lipidomics and Transcriptomics Unveil Aberrant Lipid Metabolic Remodeling in Visceral and Subcutaneous Adipose Tissue under ER Stress

• DOI: 10.1021/acs.jproteome.4c00952
• Publication Date: 2025-03-11T15:34:03Z
• Authors: Ping He; Li Zhang; Peng Ma; Tianshu Xu; Zijing Wang; Li Li; Guanhua Du; Guifen Qiang; Cuiqing Liu
• Keywords: Subcutaneous fat

Endoplasmic reticulum (ER) stress is known to impair the function of visceral adipose tissue (VAT) and subcutaneous (SAT), disrupting lipid metabolism. Despite crucial role plays in regulating function, specific lipidomic alterations VAT SAT under ER remain unclear. In this study, was induced SAT, targeted transcriptomic approaches were used analyze metabolism gene expression profiles. The results revealed that exhibited a stronger response, characterized by significant increase binding immunoglobulin protein (BiP) notable disruptions, especially glycerides sterols. These disruptions marked decrease protective polyunsaturated fatty acyl species accumulation lipotoxic molecules. contrast, displayed less severe alterations. Transcriptomic analysis indicated more susceptible immune activation, inflammation, metabolic dysfunction, while primarily showed folding processes. findings underscore tissue-specific mechanisms adaptation SAT. conclusion, appears be critical target for addressing dysfunction obesity related disorders, with potential therapeutic implications managing stress-induced diseases.