Calf Fitness Associates with Early Embryo and Recipient Metabolomes and with Calf Epigenetic Marks

• DOI: 10.1021/acs.jproteome.4c00987
• Publication Date: 2025-04-10T05:20:43Z
• Authors: Isabel Gimeno; María Berdasco; Miguel L. Pato; Pascal Salvetti; Susana Carrocera; Aurora García; Enrique Gómez

We used metadata to explore the metabolic interplay between culture medium from in vitro-produced bovine embryos transferred fresh or frozen, recipient blood plasma, and calf fitness, alongside gene expression methylation lymphocytes. Principal component (PC) analysis (PCA) identified covariates that were depicted Debiased Sparse Partial Correlation networks analyzed as enriched pathways. Four PCs explained 13.77, 9.58, 7.73 5.84% variability. PC1 clustered only mother weight two embryonic metabolites. PC2, PC3 PC4 associated 10, 17, 5 features with 6, 16 2, 20, metabolites, respectively. Subsequently, expression, fitness by PCA. Three covered 100% acid-base balance, protein metabolism, Cl-, Ca2+ IGF2 IL1R1 IL4 IL12B methylation. PC2 linked H19 growth biochemical traits. growth, hydration, redox IGF2, IGF2R, IL3 methylation, H19, IGF2R expression. Gene connected embryo metabolites through via K+, HCO3- TCO2. Calf parallels early fingerprints of recipient, allowing transfer decision-making based on health.