A Comparison of the Mouse and Human Lipoproteome: Suitability of the Mouse Model for Studies of Human Lipoproteins

• DOI: 10.1021/acs.jproteome.5b00213
• Publication Date: 2015-04-20T23:29:02Z
• Authors: Scott M. Gordon; Hailong Li; Xiaoting Zhu; Amy S. Shah; L. Jason Lu; W. Sean Davidson
• Keywords: Mice, Inbred C57BL; Mice; Spectrometry, Mass, Electrospray Ionization; 0303 health sciences; 03 medical and health sciences; Models, Chemical; Proteome; Lipoproteins; Animals; Humans; Chromatography, High Pressure Liquid

Plasma levels of low density lipoproteins (LDL) and high (HDL) exhibit opposing associations with cardiovascular disease in human populations mouse models have been heavily used to derive a mechanistic understanding these relationships. In humans, recent mass spectrometry studies revealed that the plasma lipoproteome is significantly more complex than originally appreciated. This particularly true for HDL which contains some 90 distinct proteins, majority play functional roles go beyond those expected simple lipid transport. Unfortunately, remains largely uncharacterized—a significant gap given heavy reliance on model. Using gel filtration chromatography analysis targets phospholipid-bound we compared its size distribution previous, identical analysis. We identified 113 associated proteins mouse. general, protein diversity LDL ranges was similar mice versus though differences were noted. For distributions, is, whether large small particles, example, also between species. Again, however, there clear exhibited by minority may reflect metabolic Finally, correlating profiles, five closely track major protein, apolipoprotein A-I across both Thus, most minor key inflammation, innate immunity, proteolysis inhibition, vitamin provides support continued use as model many aspects lipoprotein metabolism.