ITRAQ-Based Proteomic Analysis of the Metabolic Mechanisms Behind Lipid Accumulation and Degradation during Peanut Seed Development and Postgermination

• DOI: 10.1021/acs.jproteome.6b00345
• Publication Date: 2016-09-27T11:27:08Z
• Authors: Yun Wang; Xingli Ma; Xingguo Zhang; Xiaoyan He; Hemin Li; Dangqun Cui; Dongmei Yin
• Keywords: Proteome; Protein Degradation; Isobaric labeling

Peanut seeds have a high oil content making them an important crop. During development and germination, undergo complex dynamic physiological changes. Changes in lipid metabolism underlying mechanisms during seed been studied extensively by DNA RNA sequencing; however, there are few studies on changes of proteomics peanut germination. In this study, proteomic analyses were carried out 20, 40, 60, 80 days after pollination 5, 10, 30 germination using isobaric tags for relative absolute quantitation (iTRAQ) technology to determine the protein profiles dynamics postgermination. A total 5712 8505 proteins identified, quantified, divided into 23 functional groups, largest which was metabolism-related. Further their pathways revealed initiation fatty acid accumulation at early stages flowering, while degradation occurred largely through lipoxygenase-dependent pathway. Protein expression patterns related also verified transcript levels quantitative real-time polymerase chain reaction. The proteome determined here will significantly enrich our understanding process metabolic networks development.