Comprehensive Proteomics Analysis Reveals Metabolic Reprogramming of Tumor-Associated Macrophages Stimulated by the Tumor Microenvironment

• DOI: 10.1021/acs.jproteome.6b00604
• Publication Date: 2016-11-03T19:06:29Z
• Authors: Di Liu; Cheng Chang; Ning Lu; Xing Wang; Qian Lu; Xiaojie Ren; Peng Ren; Dianyuan Zhao; Lijing Wang; Yunping Zhu; Fuchu He; Li Tang
• Keywords: Reprogramming; Tumor-associated macrophage; Tumor progression

Tumor-associated macrophages (TAMs) are major components of the tumor microenvironment. Although a role for TAMs in promoting progression has been revealed, differentiation mechanisms and intrinsic signals regulated by microenvironment remain unclear. Here we constructed an vitro cell model, TES-TAMs, which is from tumor-extract-stimulated bone-marrow-derived macrophages. We performed comparative proteomics analysis indicated that TES-TAMs possessed characteristic molecular expression TAMs. Intriguingly, signal pathways enriched up-regulated differentially expressed proteins demonstrated glycolysis metabolism reprogramming may play important TAM differentiation. found hexokinase-2, key mediator aerobic glycolysis, downstream PFKL ENO1 were remarkably increased both primary our MMTV-PyMT mice model. This phenomenon was then verified human THP-1 lines stimulated extract solution breast cancer patient. Taken together, study provides insight into induction through metabolic reprogramming.