Proteomic Analysis of Differences in Fiber Development between Wild and Cultivated Gossypium hirsutum L.

• DOI: 10.1021/acs.jproteome.7b00122
• Publication Date: 2017-07-07T07:09:42Z
• Authors: Yuan Qin; Hengling Wei; Huiru Sun; Pengbo Hao; Hantao Wang; Junji Su; Shuxun Yu
• Keywords: Gossypium; Proteome; Phenylpropanoid

Upland cotton (Gossypium hirsutum L.) is one of the world's most important fiber crops, accounting for more than 90% all production. While their wild progenitors have relatively short and coarse, often tan-colored fibers, modern cultivars possess longer, finer, stronger, whiter fiber. In this study, cultivated cottons (YU-3 TM-1) selected show significant differences on fibers at 10 days postanthesis (DPA), 20 DPA, mature stages morphological level. To explore effects domestication, reveal molecular mechanisms underlying these phenotypic differences, better inform our efforts to further enhance quality, isobaric tags relative absolute protein quantification-facilitated proteomic methods were performed developing fibers. There 6990 proteins identified; among them, 336 defined as differentially expressed between versus domesticated cotton. The down- or up-regulated in involved phenylpropanoid biosynthesis, zeatin fatty acid elongation, other processes. Association analysis transcriptome proteome showed positive correlations transcripts both DPA DPA. Differences proteomics been verified mRNA level by quantitative real-time polymerase chain reaction validated physiological biochemical levels POD (peroxidase) activity assays ZA (zeatin) content estimates. This work corroborates major pathways development demonstrates that a great potential related elongation thickening.