Ultradeep Lysine Crotonylome Reveals the Crotonylation Enhancement on Both Histones and Nonhistone Proteins by SAHA Treatment

• DOI: 10.1021/acs.jproteome.7b00380
• Publication Date: 2017-09-08T06:28:19Z
• Authors: Quan Wu; Wenting Li; Chi Wang; Pingsheng Fan; Lejie Cao; Zhiwei Wu; Fengsong Wang
• Keywords: Non-histone protein

Lysine crotonylation is a newly discovered protein post-translational modification and was reported to share transferases deacylases with lysine acetylation. The acetyltransferase p300 also contain crotonyltransferase activity, class I histone deacetylases were demonstrated be the major decrotonylases. However, decrotonylases for nonhistone proteins are unclear. Moreover, because of lack high-quality pan-antibodies, large-scale analysis crotonylome still remains challenge. In this work, we comprehensively studied on both histones upon SAHA treatment dramatically identified 10 163 sites in A549 cells. This first identification tens thousands largest data set up now. parallel-reaction-monitoring-based experiment performed validation, which presented highly consistent results SILAC experiments. By intensive bioinformatic analysis, it found that participates wide range biological functions processes. More importantly, revealed acetylation levels most core number as well some known substrates IIa IIb HDACs up-regulated after treatment. These suggest may have decrotonylation inhibitory activities by inhibiting HDACs.