Anti-Cancer Effects of Emodin on HepG2 Cells as Revealed by 1H NMR Based Metabolic Profiling

• DOI: 10.1021/acs.jproteome.8b00029
• Publication Date: 2018-04-20T10:35:07Z
• Authors: Yue-Xiao Xing; Ming-Hui Li; Liang Tao; Ling-Yu Ruan; Wei Hong; Cheng Chen; Wen-Long Zhao; Han Xu; Jian-Feng Chen; Jun-Song Wang
• Keywords: Polygonum

Hepatic carcinoma is one of the most common cancers in world, with a high incidence. Emodin an anthraquinone derived from Polygonum multiflorum Thunb, possessing anti-cancer activity. The purpose this study to investigate effect different dosages emodin on HepG2 cells using 1H NMR based metabolic approach complemented qRT-PCR and flow cytometry identify potential markers discover targets explore underlying mechanism. can dose-dependently inhibit growth cells, perturb cell cycle progression, down-regulate expression genes proteins related glycolysis, trigger intracellular ROS generation. Orthogonal signal correction partial least-squares discriminant analysis (OSC-PLS-DA) correlation network data showed significant changes many endogenous metabolites after exposure concerning oxidative stress disturbances amino acid energy metabolism. These findings are helpful understand mechanism provide theoretical basis for its future application development.