Development of a Parallel Reaction Monitoring-MS Method To Quantify IGF Proteins in Dogs and a Case of Nonislet Cell Tumor Hypoglycemia

• DOI: 10.1021/acs.jproteome.8b00259
• Publication Date: 2018-10-30T17:22:37Z
• Authors: Alberto Valdés; Moira Lewitt; Erica Wiss; Margareta Ramström; Emma M. Strage
• Keywords: 0303 health sciences; 03 medical and health sciences; Dogs; Insulin-Like Growth Factor II; Somatomedins; Neoplasms; Methods; Animals; Humans; Reproducibility of Results; Hypoglycemia

Nonislet-cell tumor hypoglycemia (NICTH) is a rare paraneoplastic phenomenon well described in dogs and humans. Tumors associated with NICTH secrete incompletely processed forms of insulin-like growth factor-II (IGF-II), commonly named big IGF-II. These have increased bioavailability interact the insulin IGF-I receptor causing growth-promoting effects. Immunoassays designed for human samples been used to measure canine -II, but they possess some limitations. In addition, there are no validated methods measurement IGF-II dogs. present study, targeted parallel reaction monitoring MS-based method previously developed cats has optimized applied simultaneously quantify serum levels IGF-I, IGF-II, IGFBP-3, first time, This allows absolute quantification IGF proteins using mixture QPrEST The possesses good linearity repeatability evaluate IGF-system dog syndrome. this dog, decreased by 80% IGFBP-3 approximately 20- 4-times, respectively, after removal tumor.