Integrated Lipidomics and Transcriptomics Characterization upon Aging-Related Changes of Lipid Species and Pathways in Human Bone Marrow Mesenchymal Stem Cells

• DOI: 10.1021/acs.jproteome.8b00936
• Publication Date: 2019-03-04T08:50:18Z
• Authors: Xin Lu; Yue Chen; Huiyu Wang; Yunfan Bai; Jianxiang Zhao; Xiaohan Zhang; Li Liang; Yang Chen; Chenfei Ye; Yiqun Li; Yi Zhang; Yu Li; Ting Ma
• Keywords: Adult; Male; 0301 basic medicine; Aging; Principal Component Analysis; Sphingolipids; Phosphatidylethanolamines; Primary Cell Culture; Phosphatidic Acids; Bone Marrow Cells; Mesenchymal Stem Cells; Phosphatidylglycerols; Phosphatidylserines; Middle Aged; Lipid Metabolism; Phosphatidylinositols; 03 medical and health sciences; Lipidomics; Phosphatidylcholines; Humans; Transcriptome

Aberrant differentiations of bone mesenchymal stem cells (BMSCs) have proved to be associated with the occurrence senile osteoporosis. However, mechanisms this phenomenon relative abnormal lipid metabolism remain unclear. This study was conducted characterize lipidomics alterations during BMSC passaging, aiming at uncovering aging-related that may play an important role in aberrant BMSCs. Principal component analysis presented sequential passaging. The majority glycerophospholipids, including phosphatidylcholines, phosphatidylethanolamines, phosphatidylglycerols, as well sphingolipids, revealed significant elevations, whereas others, phosphatidic acids, phosphatidylinositols, and phosphatidylserines, decreases aged cells. Double-bond equivalent versus carbon number plots demonstrated changing trends significances lipids passaging were chain length degree unsaturation. In correlation networks, scattering patterns categories suggested category-related metabolic independence potential conversion among phosphatidylserines. lipid–enzyme integrated pathway indicated activated from acids CDP-diacylglycerol phosphatidylinositols sphingosine ceramides sphingomyelins conversions species described responses potentially induced aging.