Microstreaming inside Model Cells Induced by Ultrasound and Microbubbles

• DOI: 10.1021/acs.langmuir.0c00536
• Publication Date: 2020-05-14T22:37:55Z
• Authors: Valerio Pereno; Junjun Lei; Dario Carugo; Eleanor Stride
• Keywords: 570; 0303 health sciences; 03 medical and health sciences; 610

Studies on the bioeffects produced by ultrasound and microbubbles have focused primarily transport in bulk tissue, drug uptake individual cells, disruption of biological membranes. Relatively little is known about physical perturbations fluid dynamics intracellular environment during exposure. To investigate this, a custom acoustofluidic chamber was designed to expose model form giant unilamellar vesicles, microbubbles. The motion fluorescent tracer beads within lumen vesicles tracked exposure laminar flow (∼1 mm s–1), (1 MHz, ∼150 kPa, 60 s), phospholipid-coated microbubbles, alone combination. decouple effects exposure, system also modeled numerically using boundary-driven streaming field equations. Both experimental numerical results indicate that all conditions internal vesicles. Ultrasound an average bead velocity 6.5 ± 1.3 μm/s, which increased 8.5 3.8 μm/s presence compared 12 0.12 under flow. Further research forces mammalian cells associated vitro vivo are required fully determine implications for safety and/or therapy.