Surface Coating Structure and Its Interaction with Cytochrome c in EG6-Coated Nanoparticles Varies with Surface Curvature

• DOI: 10.1021/acs.langmuir.0c00681
• Publication Date: 2020-04-17T16:23:22Z
• Authors: Clyde A. Daly; Caley Allen; Nikita Rozanov; Gene Chong; Eric S. Melby; Thomas R. Kuech; Samuel E. Lohse; Catherine J. Murphy; Joel A. Pedersen; Rigoberto Hernandez
• Keywords: Cytochromes c; Metal Nanoparticles; Gold; Ligands; 3-Mercaptopropionic Acid; 01 natural sciences; 0104 chemical sciences

The composition, orientation, and conformation of proteins in biomolecular coronas acquired by nanoparticles biological media contribute to how they are identified a cell. While numerous studies have investigated protein composition coronas, relatively little detail is known about the nanoparticle surface influences orientation associated with them. We previously showed that peripheral membrane cytochrome c adopts preferred poses relative negatively charged 3-mercaptopropionic acid (MPA)–gold (AuNPs). Here, we employ molecular dynamics simulations complementary experiments establish also assumes upon association functionalized an uncharged ligand, specifically ω-(1-mercaptounde-11-cyl)hexa(ethylene glycol) (EG6). find display EG6 ligands sensitive curvature surface—and, consequently, effective diameter nearly spherical core—which turn affects c.