Self-Assembly of Mammalian-Cell Membranes on Bioelectronic Devices with Functional Transmembrane Proteins

• DOI: 10.1021/acs.langmuir.0c00804
• Publication Date: 2020-05-10T23:18:48Z
• Authors: Han-Yuan Liu; Anna-Maria Pappa; Aimie Pavia; Charalampos Pitsa...; Quentin Thiburce; Alberto Salleo; Róisín M. Owens; Susan Daniel
• Keywords: Cell membrane; Biomolecule; Transmembrane channels

Transmembrane proteins (TMPs) regulate processes occurring at the cell surface and are essential gatekeepers of information flow across membrane. TMPs difficult to study, given complex environment membrane its influence on protein conformation, mobility, biomolecule interaction, activity. For first time, we create mammalian biomembranes supported a transparent, electrically conducting polymer surface, which enables dual electrical optical monitoring TMP function in native environment. Mammalian plasma vesicles containing ATP-gated P2X2 ion channels self-assemble biocompatible cushion that transduces changes flux during ATP exposure. This platform maintains complexity membrane, fluidity constituents, orientation critical channel function. We demonstrate dual-modality readout using microscopy characterize mobility by single-particle tracking sensing gating impedance spectroscopy. measurement activity important for pain sensing, neurological activity, sensory raises new possibilities drug screening biosensing applications.